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Distribution of parathyroid hormone-stimulated adenylate cyclase in plasma membranes of cells of the kidney cortex

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Summary

Free flow electrophoresis was employed to separate renal cortical plasma membranes into luminal (brush border microvilli) and contraluminal (basal-lateral membrane) fractions. During the separation adenylate cyclase activity was found to parallel the activity of Na+−K+-activated ATPase, an enzyme which is present in contraluminal but not in luminal membranes. In the basal-lateral membrane fraction the specific activities of adenylate cyclase and Na+−K+-activated ATPase were 4.4 and 4.6 times greater, respectively, than in the brush border fraction.

The adenylate cyclase of the basal-lateral membrane fraction was specifically stimulated by parathyroid hormone which maximally increased enzyme activity eightfold. The biologically active (1–34) peptide fragment of parathyroid hormone produced a 350% increase in adenylate cyclase activity. In contrast, calcitonin, epinephrine and vasopressin maximally stimulated the enzyme by only 55, 35 and 30%, respectively.

These results indicate that adenylate cyclase, specifically stimulated by parathyroid hormone, is distributed preferentially in the contraluminal region of the plasma membrane of renal cortical epithelial cells.

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Authors and Affiliations

  1. Department of Physiology and Biophysics, Mount Sinai Medical and Graduate Schools of the City University of New York, 10029, New York, N.Y.

    Linda J. Shlatz, Irving L. Schwartz, Evamaria Kinne-Saffran & Rolf Kinne

  2. Max-Planck-Institut für Biophysik, Frankfurt, Germany

    Linda J. Shlatz, Irving L. Schwartz, Evamaria Kinne-Saffran & Rolf Kinne

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  1. Linda J. Shlatz
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  2. Irving L. Schwartz
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  3. Evamaria Kinne-Saffran
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  4. Rolf Kinne
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Shlatz, L.J., Schwartz, I.L., Kinne-Saffran, E. et al. Distribution of parathyroid hormone-stimulated adenylate cyclase in plasma membranes of cells of the kidney cortex. J. Membrain Biol. 24, 131–144 (1975). https://doi.org/10.1007/BF01868619

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  • DOI: https://doi.org/10.1007/BF01868619

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